Review



single cell sequence specific amplification kit  (Vazyme Biotech Co)


Bioz Verified Symbol Vazyme Biotech Co is a verified supplier
Bioz Manufacturer Symbol Vazyme Biotech Co manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Vazyme Biotech Co single cell sequence specific amplification kit
    Single Cell Sequence Specific Amplification Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 94/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/single+cell+sequence+specific+amplification+kit/Discover-sc+Single+Cell+WGA+Kit/pm39536623-106-22-28
    Average 94 stars, based on 40 article reviews
    single cell sequence specific amplification kit - by Bioz Stars, 2026-09
    94/100 stars

    Images

    Related Articles

    Single Cell:

    Article Title: Enhancing porcine oocyte quality and embryo development through natural antioxidants.
    Article Snippet: During fetal development, primordial oocytes maintain their developmental potential through a ROS-minimizing metabolic mechanism.. Maturation increases ROS levels, causing stress and damage, which are countered by in vivo antioxidants.. In vitro maturation (IVM) worsens this due to fewer antioxidant presence and medium factors.

    Article Title: DBP Exposure Affects Oocyte Fertilization Via Extracellular Vesicles-Derived miR-116-5p in Ovarian Granulosa Cells Through Downregulating FOXO3a Expression.
    Article Snippet: Mono-butyl phthalate (MBP), the metabolite of dibutyl phthalate (DBP), is the most abundant phthalate metabolite found in Chinese women.. Extracellular vesicles (EVs) are nanoscale lipid bilayer particles produced by extensive kinds of cells, serving a key role in intercellular communication.. Extracellular vesicle miRNAs (EV-miRNAs) in follicular fluid (FF) have been evidenced to be associated with female reproductive health.

    Article Title: GATAD2B is required for pre-implantation embryonic development by regulating zygotic genome activation.
    Article Snippet: Following three washes with TBS-T, protein bands were visualized using the ECL Plus Western Blotting Detection System (GE Healthcare). .. cDNA from embryos was obtained using a Single Cell Sequence Specific Amplification Kit (P621; Vazyme) according to the manufacturer's instructions. ..

    Article Title: Recryopreservation impairs blastocyst implantation potential via activated endoplasmic reticulum stress pathway and induced apoptosis
    Article Snippet: .. Total RNA extraction and amplification were performed using Single Cell Sequence Specific Amplification Kit (Cat: P621‐01, Vazyme). .. Quantitative RT‐PCR was performed using ChamQ Universal SYBR qPCR Master Mix (Cat: Q711‐02, Vazyme) on the LightCycler 480 system (Roche).

    Article Title: Single-cell RNA sequencing illuminates the ontogeny, conservation and diversification of cartilaginous and bony fish lymphocytes
    Article Snippet: .. We performed RNA isolation and reverse transcription using TRIzol reagent (Invitrogen, 15596026), and single cell sequence-specific amplification kit (Vazyme, P621-01) according to the manufacturer’s instructions. .. QuantiFast SYBR Green RT-PCR Kit (Qiangen, 204156) was used for quantitative real-time PCR with the Roche LightCycler® 96 Instrument.

    Article Title: hnRNPU is required for spermatogonial stem cell pool establishment in mice.
    Article Snippet: Total RNAwas prepared from testes using TRIzol reagent (Invitrogen, 15596-025) following themanufacturer’s procedure. .. Formicrocellular RNA extraction and reverse transcription: RNA from isolated germ cells from neonatal control and Vasa-Cre-cKO testes were extracted and reverse transcribed using a single-cell sequence-specific amplification kit (P621, Vazyme). .. The operation steps are carried out under the manufacturer’s instructions. qRT-PCRwas performed using SYBR green master mix with gene-specific primer sets in a Step One Plus machine (Applied Biosystems, 4309155) on an equal amount of total RNA from testes of each genotype and analyzed using the 2 DDCt method with GAPDH as an internal control.

    Article Title: Transcription factor Hoxb5 reveals the unidirectional hierarchy of hematopoietic stem cell pool.
    Article Snippet: .. After sorting, the cells were subjected to amplification using a single cell sequence specific amplification kit (Vazyme, P261, Version 22.1). .. The pre-amplified products were subsequently analyzed for Hoxb5 gene expression levels using qPCR (Vazyme, Q711, Version 22.1), with Gapdh serving as the reference gene.

    Sequencing:

    Article Title: Enhancing porcine oocyte quality and embryo development through natural antioxidants.
    Article Snippet: During fetal development, primordial oocytes maintain their developmental potential through a ROS-minimizing metabolic mechanism.. Maturation increases ROS levels, causing stress and damage, which are countered by in vivo antioxidants.. In vitro maturation (IVM) worsens this due to fewer antioxidant presence and medium factors.

    Article Title: DBP Exposure Affects Oocyte Fertilization Via Extracellular Vesicles-Derived miR-116-5p in Ovarian Granulosa Cells Through Downregulating FOXO3a Expression.
    Article Snippet: Mono-butyl phthalate (MBP), the metabolite of dibutyl phthalate (DBP), is the most abundant phthalate metabolite found in Chinese women.. Extracellular vesicles (EVs) are nanoscale lipid bilayer particles produced by extensive kinds of cells, serving a key role in intercellular communication.. Extracellular vesicle miRNAs (EV-miRNAs) in follicular fluid (FF) have been evidenced to be associated with female reproductive health.

    Article Title: GATAD2B is required for pre-implantation embryonic development by regulating zygotic genome activation.
    Article Snippet: Following three washes with TBS-T, protein bands were visualized using the ECL Plus Western Blotting Detection System (GE Healthcare). .. cDNA from embryos was obtained using a Single Cell Sequence Specific Amplification Kit (P621; Vazyme) according to the manufacturer's instructions. ..

    Article Title: Recryopreservation impairs blastocyst implantation potential via activated endoplasmic reticulum stress pathway and induced apoptosis
    Article Snippet: .. Total RNA extraction and amplification were performed using Single Cell Sequence Specific Amplification Kit (Cat: P621‐01, Vazyme). .. Quantitative RT‐PCR was performed using ChamQ Universal SYBR qPCR Master Mix (Cat: Q711‐02, Vazyme) on the LightCycler 480 system (Roche).

    Article Title: Single-cell RNA sequencing illuminates the ontogeny, conservation and diversification of cartilaginous and bony fish lymphocytes
    Article Snippet: .. We performed RNA isolation and reverse transcription using TRIzol reagent (Invitrogen, 15596026), and single cell sequence-specific amplification kit (Vazyme, P621-01) according to the manufacturer’s instructions. .. QuantiFast SYBR Green RT-PCR Kit (Qiangen, 204156) was used for quantitative real-time PCR with the Roche LightCycler® 96 Instrument.

    Article Title: hnRNPU is required for spermatogonial stem cell pool establishment in mice.
    Article Snippet: Total RNAwas prepared from testes using TRIzol reagent (Invitrogen, 15596-025) following themanufacturer’s procedure. .. Formicrocellular RNA extraction and reverse transcription: RNA from isolated germ cells from neonatal control and Vasa-Cre-cKO testes were extracted and reverse transcribed using a single-cell sequence-specific amplification kit (P621, Vazyme). .. The operation steps are carried out under the manufacturer’s instructions. qRT-PCRwas performed using SYBR green master mix with gene-specific primer sets in a Step One Plus machine (Applied Biosystems, 4309155) on an equal amount of total RNA from testes of each genotype and analyzed using the 2 DDCt method with GAPDH as an internal control.

    Article Title: Transcription factor Hoxb5 reveals the unidirectional hierarchy of hematopoietic stem cell pool.
    Article Snippet: .. After sorting, the cells were subjected to amplification using a single cell sequence specific amplification kit (Vazyme, P261, Version 22.1). .. The pre-amplified products were subsequently analyzed for Hoxb5 gene expression levels using qPCR (Vazyme, Q711, Version 22.1), with Gapdh serving as the reference gene.

    Amplification:

    Article Title: Enhancing porcine oocyte quality and embryo development through natural antioxidants.
    Article Snippet: During fetal development, primordial oocytes maintain their developmental potential through a ROS-minimizing metabolic mechanism.. Maturation increases ROS levels, causing stress and damage, which are countered by in vivo antioxidants.. In vitro maturation (IVM) worsens this due to fewer antioxidant presence and medium factors.

    Article Title: DBP Exposure Affects Oocyte Fertilization Via Extracellular Vesicles-Derived miR-116-5p in Ovarian Granulosa Cells Through Downregulating FOXO3a Expression.
    Article Snippet: Mono-butyl phthalate (MBP), the metabolite of dibutyl phthalate (DBP), is the most abundant phthalate metabolite found in Chinese women.. Extracellular vesicles (EVs) are nanoscale lipid bilayer particles produced by extensive kinds of cells, serving a key role in intercellular communication.. Extracellular vesicle miRNAs (EV-miRNAs) in follicular fluid (FF) have been evidenced to be associated with female reproductive health.

    Article Title: GATAD2B is required for pre-implantation embryonic development by regulating zygotic genome activation.
    Article Snippet: Following three washes with TBS-T, protein bands were visualized using the ECL Plus Western Blotting Detection System (GE Healthcare). .. cDNA from embryos was obtained using a Single Cell Sequence Specific Amplification Kit (P621; Vazyme) according to the manufacturer's instructions. ..

    Article Title: Recryopreservation impairs blastocyst implantation potential via activated endoplasmic reticulum stress pathway and induced apoptosis
    Article Snippet: .. Total RNA extraction and amplification were performed using Single Cell Sequence Specific Amplification Kit (Cat: P621‐01, Vazyme). .. Quantitative RT‐PCR was performed using ChamQ Universal SYBR qPCR Master Mix (Cat: Q711‐02, Vazyme) on the LightCycler 480 system (Roche).

    Article Title: Single-cell RNA sequencing illuminates the ontogeny, conservation and diversification of cartilaginous and bony fish lymphocytes
    Article Snippet: .. We performed RNA isolation and reverse transcription using TRIzol reagent (Invitrogen, 15596026), and single cell sequence-specific amplification kit (Vazyme, P621-01) according to the manufacturer’s instructions. .. QuantiFast SYBR Green RT-PCR Kit (Qiangen, 204156) was used for quantitative real-time PCR with the Roche LightCycler® 96 Instrument.

    Article Title: hnRNPU is required for spermatogonial stem cell pool establishment in mice.
    Article Snippet: Total RNAwas prepared from testes using TRIzol reagent (Invitrogen, 15596-025) following themanufacturer’s procedure. .. Formicrocellular RNA extraction and reverse transcription: RNA from isolated germ cells from neonatal control and Vasa-Cre-cKO testes were extracted and reverse transcribed using a single-cell sequence-specific amplification kit (P621, Vazyme). .. The operation steps are carried out under the manufacturer’s instructions. qRT-PCRwas performed using SYBR green master mix with gene-specific primer sets in a Step One Plus machine (Applied Biosystems, 4309155) on an equal amount of total RNA from testes of each genotype and analyzed using the 2 DDCt method with GAPDH as an internal control.

    Article Title: Transcription factor Hoxb5 reveals the unidirectional hierarchy of hematopoietic stem cell pool.
    Article Snippet: .. After sorting, the cells were subjected to amplification using a single cell sequence specific amplification kit (Vazyme, P261, Version 22.1). .. The pre-amplified products were subsequently analyzed for Hoxb5 gene expression levels using qPCR (Vazyme, Q711, Version 22.1), with Gapdh serving as the reference gene.

    Reverse Transcription:

    Article Title: DBP Exposure Affects Oocyte Fertilization Via Extracellular Vesicles-Derived miR-116-5p in Ovarian Granulosa Cells Through Downregulating FOXO3a Expression.
    Article Snippet: Mono-butyl phthalate (MBP), the metabolite of dibutyl phthalate (DBP), is the most abundant phthalate metabolite found in Chinese women.. Extracellular vesicles (EVs) are nanoscale lipid bilayer particles produced by extensive kinds of cells, serving a key role in intercellular communication.. Extracellular vesicle miRNAs (EV-miRNAs) in follicular fluid (FF) have been evidenced to be associated with female reproductive health.

    Article Title: Single-cell RNA sequencing illuminates the ontogeny, conservation and diversification of cartilaginous and bony fish lymphocytes
    Article Snippet: .. We performed RNA isolation and reverse transcription using TRIzol reagent (Invitrogen, 15596026), and single cell sequence-specific amplification kit (Vazyme, P621-01) according to the manufacturer’s instructions. .. QuantiFast SYBR Green RT-PCR Kit (Qiangen, 204156) was used for quantitative real-time PCR with the Roche LightCycler® 96 Instrument.

    Article Title: hnRNPU is required for spermatogonial stem cell pool establishment in mice.
    Article Snippet: Total RNAwas prepared from testes using TRIzol reagent (Invitrogen, 15596-025) following themanufacturer’s procedure. .. Formicrocellular RNA extraction and reverse transcription: RNA from isolated germ cells from neonatal control and Vasa-Cre-cKO testes were extracted and reverse transcribed using a single-cell sequence-specific amplification kit (P621, Vazyme). .. The operation steps are carried out under the manufacturer’s instructions. qRT-PCRwas performed using SYBR green master mix with gene-specific primer sets in a Step One Plus machine (Applied Biosystems, 4309155) on an equal amount of total RNA from testes of each genotype and analyzed using the 2 DDCt method with GAPDH as an internal control.

    RNA Extraction:

    Article Title: Recryopreservation impairs blastocyst implantation potential via activated endoplasmic reticulum stress pathway and induced apoptosis
    Article Snippet: .. Total RNA extraction and amplification were performed using Single Cell Sequence Specific Amplification Kit (Cat: P621‐01, Vazyme). .. Quantitative RT‐PCR was performed using ChamQ Universal SYBR qPCR Master Mix (Cat: Q711‐02, Vazyme) on the LightCycler 480 system (Roche).

    Article Title: hnRNPU is required for spermatogonial stem cell pool establishment in mice.
    Article Snippet: Total RNAwas prepared from testes using TRIzol reagent (Invitrogen, 15596-025) following themanufacturer’s procedure. .. Formicrocellular RNA extraction and reverse transcription: RNA from isolated germ cells from neonatal control and Vasa-Cre-cKO testes were extracted and reverse transcribed using a single-cell sequence-specific amplification kit (P621, Vazyme). .. The operation steps are carried out under the manufacturer’s instructions. qRT-PCRwas performed using SYBR green master mix with gene-specific primer sets in a Step One Plus machine (Applied Biosystems, 4309155) on an equal amount of total RNA from testes of each genotype and analyzed using the 2 DDCt method with GAPDH as an internal control.

    Isolation:

    Article Title: Single-cell RNA sequencing illuminates the ontogeny, conservation and diversification of cartilaginous and bony fish lymphocytes
    Article Snippet: .. We performed RNA isolation and reverse transcription using TRIzol reagent (Invitrogen, 15596026), and single cell sequence-specific amplification kit (Vazyme, P621-01) according to the manufacturer’s instructions. .. QuantiFast SYBR Green RT-PCR Kit (Qiangen, 204156) was used for quantitative real-time PCR with the Roche LightCycler® 96 Instrument.

    Article Title: hnRNPU is required for spermatogonial stem cell pool establishment in mice.
    Article Snippet: Total RNAwas prepared from testes using TRIzol reagent (Invitrogen, 15596-025) following themanufacturer’s procedure. .. Formicrocellular RNA extraction and reverse transcription: RNA from isolated germ cells from neonatal control and Vasa-Cre-cKO testes were extracted and reverse transcribed using a single-cell sequence-specific amplification kit (P621, Vazyme). .. The operation steps are carried out under the manufacturer’s instructions. qRT-PCRwas performed using SYBR green master mix with gene-specific primer sets in a Step One Plus machine (Applied Biosystems, 4309155) on an equal amount of total RNA from testes of each genotype and analyzed using the 2 DDCt method with GAPDH as an internal control.

    Control:

    Article Title: hnRNPU is required for spermatogonial stem cell pool establishment in mice.
    Article Snippet: Total RNAwas prepared from testes using TRIzol reagent (Invitrogen, 15596-025) following themanufacturer’s procedure. .. Formicrocellular RNA extraction and reverse transcription: RNA from isolated germ cells from neonatal control and Vasa-Cre-cKO testes were extracted and reverse transcribed using a single-cell sequence-specific amplification kit (P621, Vazyme). .. The operation steps are carried out under the manufacturer’s instructions. qRT-PCRwas performed using SYBR green master mix with gene-specific primer sets in a Step One Plus machine (Applied Biosystems, 4309155) on an equal amount of total RNA from testes of each genotype and analyzed using the 2 DDCt method with GAPDH as an internal control.

    other:

    Article Title: Single-cell multi-omics profiling of human preimplantation embryos identifies cytoskeletal defects during embryonic arrest.
    Article Snippet: Human in vitro fertilized embryos exhibit low developmental capabilities, and the mechanisms that underlie embryonic arrest remain unclear.. Here using a single-cell multi-omics sequencing approach, we simultaneously analysed alterations in the transcriptome, chromatin accessibility and the DNA methylome in human embryonic arrest due to unexplained reasons.. Arrested embryos displayed transcriptome disorders, including a distorted microtubule cytoskeleton, increased genomic instability and impaired glycolysis, which were coordinated with multiple epigenetic reprogramming defects.



    Similar Products

    94
    Vazyme Biotech Co single cell sequence specific amplification kit
    Single Cell Sequence Specific Amplification Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/single+cell+sequence+specific+amplification+kit/Discover-sc+Single+Cell+WGA+Kit/pm39536623-106-22-28
    Average 94 stars, based on 1 article reviews
    single cell sequence specific amplification kit - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    Image Search Results